Phase III interlaboratory study of FETAX part 3. FETAX validation using 12 compounds with and without an exogenous metabolic activation system

1999 ◽  
Vol 19 (6) ◽  
pp. 447-472 ◽  
Author(s):  
John A. Bantle ◽  
Robert A. Finch ◽  
Douglas J. Fort ◽  
Enos L. Stover ◽  
Mendi Hull ◽  
...  
Keyword(s):  
Metabolic Activation ◽  
Interlaboratory Study ◽  
Phase Iii ◽  
Activation System ◽  
Exogenous Metabolic Activation

OPTIMIZATION OF AN EXOGENOUS METABOLIC ACTIVATION SYSTEM FOR FETAX. II. PRELIMINARY EVALUATION

2001 ◽  
Vol 24 (2) ◽  
pp. 117-127 ◽  
Author(s):  
Douglas J. Fort ◽  
Robert L. Rogers ◽  
Robbin R. Paul ◽  
Enos L. Stover ◽  
Robert A. Finch
Keyword(s):  
Metabolic Activation ◽  
Preliminary Evaluation ◽  
Activation System ◽  
Exogenous Metabolic Activation

scholarly journals Comparative Toxigenicity and Associated Mutagenicity of Aspergillus fumigatus and Aspergillus flavus Group Isolates Collected from the Agricultural Environment

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 458
Author(s):  
Caroline Lanier ◽  
David Garon ◽  
Natacha Heutte ◽  
Valérie Kientz ◽  
Véronique André
Keyword(s):  
Ames Test ◽  
Metabolic Activation ◽  
Agricultural Environment ◽  
Activation System ◽  
Mutagenic Response ◽  
Exogenous Metabolic Activation ◽  
Potential Impact ◽  
Potential Interactions ◽  
Aspergillus Flavus Group ◽  
Higher Doses

The mutagenic patterns of A. flavus, A. parasiticus and A. fumigatus extracts were evaluated. These strains of toxigenic Aspergillus were collected from the agricultural environment. The Ames test was performed on Salmonella typhimurium strains TA98, TA100 and TA102, without and with S9mix (exogenous metabolic activation system). These data were compared with the mutagenicity of the corresponding pure mycotoxins tested alone or in reconstituted mixtures with equivalent concentrations, in order to investigate the potential interactions between these molecules and/or other natural metabolites. At least 3 mechanisms are involved in the mutagenic response of these aflatoxins: firstly, the formation of AFB1-8,9-epoxide upon addition of S9mix, secondly the likely formation of oxidative damage as indicated by significant responses in TA102, and thirdly, a direct mutagenicity observed for higher doses of some extracts or associated mycotoxins, which does not therefore involve exogenously activated intermediates. Besides the identified mycotoxins (AFB1, AFB2 and AFM1), additional “natural” compounds contribute to the global mutagenicity of the extracts. On the other hand, AFB2 and AFM1 modulate negatively the mutagenicity of AFB1 when mixed in binary or tertiary mixtures. Thus, the evaluation of the mutagenicity of “natural” mixtures is an integrated parameter that better reflects the potential impact of exposure to toxigenic Aspergilli.

Assessing the Efficacy of an Aroclor 1254–Induced Exogenous Metabolic Activation System for Fetax

1991 ◽  
Vol 14 (1-2) ◽  
pp. 143-160 ◽  
Author(s):  
Douglas J. Fort ◽  
James R. Rayburn ◽  
Donna J. Deyoung ◽  
John A. Bantle
Keyword(s):  
Metabolic Activation ◽  
Aroclor 1254 ◽  
Activation System ◽  
Exogenous Metabolic Activation

Human embryonic cell growth assay for teratogens with or without metabolic activation system using microplate

1988 ◽  
Vol 8 (5) ◽  
pp. 265-272 ◽  
Author(s):  
Toshie Tsuchiya ◽  
Atsuko Matuoka ◽  
Setsuko Sekita ◽  
Takuzo Hisano ◽  
Atsushi Takahashi ◽  
...  
Keyword(s):  
Cell Growth ◽  
Metabolic Activation ◽  
Embryonic Cell ◽  
Activation System ◽  
Growth Assay ◽  
Human Embryonic Cell
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